Regulation of gene expression through lncRNAs hos Institut for Plante- og Miljøvidenskab - Københavns Universitet

WANTED: Genome Engineer

Bachelor thesis opportunity for KU students at Marquardt lab:

“Regulation of gene expression through lncRNAs”

Gene regulation is a dynamic process involving various molecular mechanisms. One of the regulatory mechanisms is enigmatic gene repression through the act of RNA polymerase II transcription (RNAPII). We are interested in the ‘’dark’’ site of RNAPII pervasive transcription of non-coding DNA resulting in long-non-coding RNAs (lncRNAs), as a molecular mechanism of environmental change in plants.  

lncRNA.jpg

Your role:

To shed light on this elusive process, you will investigate functional effects of lncRNAs located upstream of genes involved in plant developmental processes. You will be trained in cutting-edge molecular plant science research. For example, you will generate mutant plants to disturb the lncRNAs pervasive effect utilizing CRISPR-Cas9 method. You will select these mutants using fluorescence microscope and sequencing. You will determine the effect of this deletion mutants on gene expression using qPCR. Your project targets the dissection of mechanistic principles of tandem transcription interference using genetics and biochemistry. A likely outcome of your project will be a functional characterization of lncRNAs and their involvement in regulation of gene expression in plants.

 

Please contact Sebastian or Katerina for further information

Stop by office in CPSC 782-11-2-00-F, or email:

Sebastian Marquardt (sebastian.marquardt@plen.ku.dk)

            Katerina Adamusova (mus@plen.ku.dk)

Bemærk: Når du ansøger specialeopslag, er der særlige krav, der skal opfyldes, for at du kan inkludere det som en del af dit uddannelsesforløb. For nærmere information omkring godkendelseskriterierne, kontakt venligst din studievejleder ud fra følgende oversigt. Studie- og Karrierevejledning

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