This is a plant molecular biology project focusing on tandem Transcriptional Interference (tTI). During tTI upstream transcription event elongates and suppresses the downstream transcription event. As a result, the downstream gene doesn’t have a functional transcript, and therefore it doesn’t produce protein. Our goal is to identify what genes and chromatin marks control that process.
In that project, we suggest you apply a genetic approach to identify what genes are involved in tTI. A good example of tTI was found in the qua1-1 line. In the qua1-1 line, there is a t-DNA insertion upstream of the QUA1 gene. Transcription starts from the t-DNA insertion and goes through the QUA1 gene, making a read-through transcript. We can see that the absence of functional QUA1 transcript results in dwarfed growth and ruthenium red staining of dark-grown qua1-1 hypocotyls.
You will cross qua1-1 plants with gene candidates and genotype plants and perform ruthenium red staining to see if the gene candidate was able to restore the wild-type phenotype.
If we see the restoration of phenotype in double mutant hypocotyls, we can say that the mutated gene candidate disrupted TI, which means that in a qua1-1 single mutant, this gene could support tTI.
During that project, you will learn how to grow plants on soil and media, genotype them, and do ruthenium red staining.
In case you are interested in the project contact me by email email@example.com or the head of the lab firstname.lastname@example.org