Targeting membrane repair processes in cancer cells at Biologisk Institut - Københavns Universitet

Plasma membrane repair is a fundamental process essential for cell survival upon membrane damage. Metastazising cancer cells need to have especially efficient membrane repair systems to cope with continuous mechanical stress leading to membrane damage. The Ca2+-binding protein ALG-2 has been found to be implicated in the "macro vesicle shedding" mechanism of plasma membrane repair eby mediating the assembly of the ESCRT machinery at membrane wound sites. Our recently published results with ALG-2 knock out cell line show that these cells recover less well than the parental wild type cells following membrane damage- Now, we have created knock out HeLa cancer cells by CRISPR/Cas9 lacking ALG-2. We will check the role of ALG-2 in membrane repair of metastazising cancer cells using a transwell chamber assay in order to find out whether the ALG-2 signaling pathway could be a potential target to eliminte cancer cells.

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